Sigma opioid receptors Bradykinin Apoptosis
Noscapine (0-1000 μM; 0-96 hours; rat C6 glioma cells) treatment inhibits cell viability of rat C6 glioma in vitro in a dose- and time-dependent manner. Noscapine inhibits the viability of rat C6 glioma cells with an IC
50
of 250 μM at 72 hours.
Noscapine exposure causes abnormal S-phase reentry, increases mitotic arrest and results in excessive DNA accumulation.
Cylindromatosis increases the ability of noscapine to induce mitotic arrest and apoptosis. Cylindromatosis enhances the effect of noscapine on microtubule polymerization and promotes noscapine binding to microtubules.
Cell Proliferation Assay
Cell Line: Rat C6 glioma cells Concentration: 0 μM, 0.1 μM, 1 μM, 2 μM, 10 μM, 50 μM, 100 μM, 1000 μM Incubation Time: 0 hours, 12 hours, 24 hours, 48 hours, 72 hours, 96 hours Result: Inhibited cell viability of rat C6 glioma in vitro in a dose- and time-dependent manner.Noscapine (300?mg/kg; ora1 gavage; daily; for 15 days; athymic female mice) treatment reduces tumor growth in mice.