Benproperine phosphate (20-120 μM; for 24?hours) inhibits cell viability in a dose-dependent manner.
Benproperine phosphate (10 μM; for 24?hours) significantly inhibits the migration of various types of cancer cells and inhibits the migration and invasion of DLD-1, AsPC-1 cells with IC
50
values of 1-2?μM. Benproperine phosphate (10 μM; for 24?hours) does not affect cortactin-rich lamellipodium in MCF-10A cells.
Cell Viability Assay
Cell Line: DLD-1, AsPC-1, CFPAC-1, A375P, A375P, MDA-MB-231, DU145, DU145 cancer cells Concentration: 20, 40, 60, 80, 100, 120 μM Incubation Time: For 24?hours Result: Inhibited cell viability in a dose-dependent manner.
Benproperine phosphate (50, 100?mg/kg; ora1 gavage; 5?days per week for 4?weeks) inhibits primary pancreatic tumor growth.
Benproperine phosphate shows a marked decrease in the lung metastasis of AsPC-1 cells (56.1% inhibition) in mouse. Benproperine phosphate significantly suppressed the liver metastasis of HCT-116 cells by 78.9% and DLD-1 cells by 78.2%.